SaOVA RNAs have been designed to produce high expression level of Ova protein with enhanced length of duration (see results). OZB saRNAs are produced by in vitro transcription. saRNAs are stabilised at the 5′ end by modified nucleotides capping (Cap1) and contain a poly(A) tail at the 3′ end. Sequences have been optimised to yield improved stability and performance.
OZB saOva RNA has been constructed based on replicons of positive-sense ((+)-RNA) viruses (Venezuelan equine encephalitis Virus (VEEV)), where the coding sequence of the viral structural proteins is replaced with that of a gene of interest (GOI, here OVA), while retaining the coding sequences of non-structural proteins (NsPs), including the viral RNA-dependent RNA polymerase. Please note that OZB saRNAs encodes also for Puromycin Resistance Cassette, which does not alter the expression of the GOI but can be used for cell selection.
- MRNA74 does not bear any additional nucleotide modifications, molecular weight: 3.30 E+06g/mol
- #MRNA75 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine), molecular weight: 3.34 E+06 g/mol
- Ready-to-use stabilised saOva puroR RNA
- mRNA length: 10234nt
- Storage: RNAs must be stored at -80°C
- It is recommend to aliquot the RNA solution for a better storage and avoid freeze/thaw cycles
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