Application

7α,25-dihydroxycholesterol-d6 has be used as an internal standard inliquid chromatography electrospray ionization tandem mass spectrometric (LC–ESI-MS/MS) analysis of human plasma.

Biochem/physiol Actions

7α,25-dihydroxycholesterol (7α,25-OHC) serves as an endogenous ligand for G-protein-coupled receptor 183 (GPR183) or Epstein-Barr virus-induced gene 2 (EBI2). It plays a key role in the migration of type 3 innate lymphoid cells (ILC3) in the small intestine and colon. 7α,25-OHC mediates immune cell migration functionality by their chemoattractant property. Inhibition of 7α,25-OHC synthesis leads to impairment in the migration of B cells.

General description

7α,25-dihydroxycholesterol (7α,25-OHC) is synthesized by the hydroxylation of cholesterol by the action of enzyme cholesterol 25-hydroxylase (CH25H) and cytochrome P450, family 7, subfamily b, polypeptide 1 (CYP7B1). The catabolic breakdown of 7α,25-OHC to bile acid precursors is catalyzed by the enzymes hydroxy-Δ-5-steroid dehydrogenase, 3β- and steroid Δ-isomerase 7 (HSD3B7). 7α,25-dihydroxycholesterol-d6 is the deuterated form of 7α,25-dihydroxycholesterol.

Packaging

5 mL Amber Glass Screw Cap Vial (700078P-1mg)