Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

Replaces Protein A Sepharose^® 4 Fast Flow, the first Cytiva Protein A medium for large-scale purification of antibodies. Used in routine commercial production of monoclonal antibodies Free from animal-derived components.

General description

nProtein A Sepharose^® 4 Fast Flow is native protein A coupled to Sepharose^® 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.nProtein A Sepharose^® 4 Fast Flow is native protein A coupled to the well established Sepharose^® 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose^® 4 Fast Flow is manufactured without using animal-derived components.nProtein A Sepharose^® 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose^® CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose^® 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.As member of the BioProcess media range, nProtein A Sepharose^® 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Legal Information

Sepharose is a registered trademark of Cytiva