Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

For single-step purification of native calmodulin-binding proteins Suitable for tandem affinity purification (TAP) of protein complexes Purification of calmodulin-regulated proteins from all eukaryotic cells

General description

Calmodulin Sepharose^® 4B affinity media for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells.Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD?/NADP? Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca²⁺ on separate Ca²⁺-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca²⁺ complexes are particularly stable.

Legal Information

Sepharose is a registered trademark of Cytiva