Meridian Bioscience Lyo-Ready AOF dUTP RNA/DNA qPCR Saliva 4x - 1000 Reactions

Lyo-Ready AOF dUTP DNA qPCR Saliva (MDX332) uses an advanced one-tube formulation, incorporating the latest innovations in buffer chemistry and PCR enhancers, along with an optimised concentration of recombinant antibody-mediated hot-start polymerase, dNTPs (including dUTP), and MgCl₂. Lyo-Ready AOF dUTP RNA/DNA qPCR Saliva, (MDX333) also includes reverse transcriptase and RNase inhibitor for comprehensive nucleic acid detection.

Lyo-Ready AOF dUTP DNA/RNA qPCR mixes utilise the dUTP/UDGase system to prevent carryover contamination, minimising false positives and ensuring accurate results, especially in high-throughput workflows. BSA-free and sustainable, these mixes also align with evolving regulatory trends toward in vitro systems, reducing reliance on animal-sourced reagents while offering cost-effective, room-temperature stability through their lyophilisation-ready formulation.

Saliva, a non-invasive and easily accessible diagnostic specimen, is gaining attention for detecting various diseases. Like serum, it contains hormones, antibodies, growth factors, enzymes, and microbes that serve as biomarkers. However, challenges arise when using saliva for molecular diagnostics due to low analyte concentrations and high levels of PCR inhibitors, which require complex sample preparation to purify DNA/RNA. Our inhibitor-tolerant Lyo-Ready mixes enable sensitive RNA/DNA detection, even in crude saliva and sputum samples, ensuring accurate results across a wide range of diagnostic applications.

• Supports the development of saliva-based screening assays, such as assays detecting COVID, influenza, RSV and tuberculosis
• Suitable for singleplex or multiplex assays detecting RNA or DNA pathogens at very low titers from saliva or sputum samples
• Validated for detection of saliva viruses and pathogens
• Offers the flexibility to remain as a liquid mix or be lyophilised, creating ambient-temperature stable assays with extended shelf life
• Highly sensitive detection of RNA/DNA, even in crude saliva and sputum samples
• Incorporates dUTP, making it ideal for high-throughput and multiplex assays
• Animal-origin free and sustainable, designed to reduce environmental impact while aligning with future eco-regulations