Meridian Bioscience Lyo-Ready AOF dUTP RNA/DNA qPCR Blood 4x - 1000 Reactions

Lyo-Ready AOF dUTP DNA qPCR Blood Mix (MDX322) uses an advanced one-tube formulation, incorporating the latest innovations in buffer chemistry and PCR enhancers, along with an optimized concentration of recombinant antibody-mediated hot-start polymerase, dNTPs (including dUTP), and MgCl₂. Lyo-Ready AOF dUTP RNA/DNA qPCR Blood Mix, (MDX323) also includes reverse transcriptase and RNase inhibitor for comprehensive nucleic acid detection.

Lyo-Ready AOF dUTP DNA/RNA qPCR mixes utilise the dUTP/UDGase system to prevent carryover contamination, minimising false positives and ensuring accurate results, especially in high-throughput workflows. BSA-free and sustainable, these mixes also align with evolving regulatory trends toward in vitro systems, reducing reliance on animal-sourced reagents while offering cost-effective, ambient-temperature stability through their lyophilisation-ready formulation.

Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for evaluating the function of vital organs (kidneys, liver, thyroid, and heart) and for diagnosing diseases such as bacterial and viral infections, cancer, cardiovascular disease, and metabolic disorders such as diabetes. However, inhibitors like immunoglobulin G, haemoglobin and lactoferrin can impact accuracy. Our inhibitor-tolerant Lyo-Ready mixes ensure sensitive RNA/DNA detection, even in crude whole blood, serum and plasma samples, providing reliable results for diverse diagnostic applications.

• Supports the development of blood-based screening assays, such as assays detecting cancer or infections
• Enables highly sensitive detection of low-copy number bacteria, viruses, and cell-free nucleic acids directly from crude whole blood, serum and plasma samples
• Validated for detection of blood pathogens and liquid biopsy applications
• Offers the flexibility to remain as a liquid mix or be lyophilised, creating ambient-temperature stable assays with extended shelf life
• Highly sensitive detection of RNA/DNA, even in crude whole blood, serum and plasma samples
• Incorporates dUTP, making it ideal for high-throughput and multiplex assays
• Animal origin-free and sustainable, designed to reduce environmental impact while aligning with future eco-regulations