A variety of PCR inhibitors inherent to clinical and environmental samples can negatively impact the sensitivity and accuracy of a molecular assay. Conventionally, to overcome this, specimens undergo processing before testing. However, extraction technologies are not 100% efficient and can impact the amount of target nucleic acid available for testing. Some PCR inhibitors can also co-elute following purification, reducing the efficiency, particularly in quantitative PCR multiplex assays and may even induce false-negative assay results.
Molecular diagnostic tests are progressively moving towards lyophilised formats. Meridian’s universal Lyo-Ready Inhibitor-Tolerant qPCR/RT-qPCR formulations are ready-to-use quantitative PCR supermixes, containing all components except primers, probes, and template for direct qPCR and RT-qPCR. They are also glycerol-free and include specialised excipients that preserve the mixture as it is exposed to various conditions including freezing, vacuum, and dehydration during lyophilisation. After lyophilisation they can be shipped and storage at ambient temperature, extended shelf-life, reduced operating steps and potential errors and increased flexibility in sample volume. They are designed for developing qPCR multiplex assays that require minimal sample processing and fast turn-around times (TAT) without compromising sensitivity, for low copy number samples.
- Ready-to-use lyo-ready qPCR master mixes engineered for amplification from crude samples without the need for complex or time-consuming extraction
- 4x concentration master mix allows for greater sample volume, simply add primers, probes and clinical samples
- Mixes can be lyophilised to create fast, highly reproducible, ambient temperature stable molecular diagnostic tests
This product has met the following criteria to qualify for the following awards: