Lyo-Ready Genotyping Direct qPCR FFPE is a glycerol-free, one tube formulation compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide variants and copy number variations.
SNPs (single nucleotide polymorphisms) or point mutations are the most common types of genetic variation and comprise the major part of phenotype diversity between individuals. SNPs can be responsible for both resistance or susceptibility to a certain disease and once individual SNPs have been identified by sequencing, they can be used for pharmacogenetics, in evaluating and predicting a patient’s response to treatment and risk of adverse events, or for diagnostics, such as in cystic fibrosis screening. Although blood is an ideal source for genotyping analysis, formalin-fixed paraffin-embedded (FFPE) tumour tissues are also widely used and constitute valuable resources for retrospective studies due to the possibility of long-term storage at room temperature. Despite the extremely time-consuming and labour-intensive workflows, success rate of genotyping assays using DNA purified from such samples remains low.
To minimise the workflow to isolate DNA from FFPE tissue, Meridian has created the Lyo-Ready Genotyping Direct qPCR FFPE, this allows digestion of the tissue in a single tube, without the need for purification, greatly reducing the risk of sample damage, loss and contamination whilst simplifying and accelerating the analysis process. Furthermore, it can be used in a liquid format or lyophilised format to create ambient-temperature stable assays, making it ideal for high throughput.
- Ultra-sensitive detection down to less than 1 copy using direct amplification protocols
- Inhibitor-tolerant to PCR inhibitors found in FFPE tissue
- Tight fluorescence clusters with clear allele discrimination, perfect for difficult SNPs identification
- Mixes can be used in liquid or lyophilised formats to extend assay shelf-life
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