Molecular diagnostic tests are progressively moving towards lyophilised. There are several advantages for this, including room temperature shipping and storage, extended shelf-life, and increased flexibility in the sample volume. In order to be compatible with drying, however, enzyme preparations must be glycerol-free and include specialised excipients that preserve the mixture as it is exposed to freeze-dry, vacuum and dehydration.
Lyo-Ready qPCR Buffer w/o Excipients is a glycerol-free qPCR reaction buffer that allows formulation optimisation to freeze-drying methods by excipients selection. In order to produce room temperature lyophilised qPCR reagents, lyo-excipients, a lyo-compatible Taq DNA polymerase (such as MDX011 or MDX015), assay-specific primers, and probes are added to Lyo-Ready Buffer for subsequent freeze-dry.
- Used to create ultra-sensitive qPCR assays
- Minimises non-specific amplification for improved assay sensitivity and reliability
- High efficiency, giving excellent performance in multiplex qPCR assays
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