Low DNA qPCR Mix is a fast, highly reproducible real-time PCR master mix that has been designed with a highly optimised buffer chemistry and chemical hot-start DNA polymerase, to deliver excellent results and very low background, allowing greater sensitivity.

Low DNA qPCR master mix contains a chemical hot-start Taq polymerase and highly optimised reaction buffer with dNTPs, MgCl₂, stabilisers, and PCR enhancers. The Taq polymerase is purified twice, before and after the addition of the chemical hot-start, this helps to remove trace amounts of DNA (known as bioburden) that come from the manufacturing of the DNA polymerase. In addition there is no bioburden that comes with antibody hot-start, making it ideal for low-copy number targets and also microbial DNA and fungal DNA detection assays.

Low DNA qPCR master mix can also be used for fast, precise and highly reproducible genotyping of sequence variants, including loci, provides higher stringency and more specific binding of the allele-specific probes, with wider and clearer separation of allele cluster.

• Reproducible detection of low-copy number templates
• Proprietary hot-start modification minimises non-specific amplification for improved assay reliability in high-throughput assays
• Consistent results between technical replicates ideal for multiplexing, gene expression analysis and genotyping
• Reliable, accurate detection of DNA and cDNA targets from a broad range of sample types