Low LOD 1-Step RT-qPCR Mix is fast, highly reproducible real-time PCR master mix that has been designed with a highly optimised buffer chemistry and hot-start DNA polymerase, to deliver excellent results and very low background, allowing greater sensitivity.
Low LOD 1-Step RT-qPCR master mix contains Taq DNA polymerase, reaction buffer, dNTP, MgCl2, stabilisers, and PCR enhancers, and a separate RNase tolerant reverse transcriptase mix. However, the dTTP in the dNTPs has been substituted for dUTP, so that Uracil DNA Glycosylase (UDGase) (MDX054) can be added to remove background PCR product contamination. This makes Low LOD 1-Step RT-qPCR ideal for developing highly sensitive RT-qPCR diagnostic tests, such as for detecting RNA and DNA viruses at very low levels in blood banks or transplant viral testing.
- Suitable for detecting purified RNA and DNA viruses at very low titers
- High efficiency, giving excellent performance in multiplex qPCR assays
- Prevents carry-over contamination
This product has met the following criteria to qualify for the following awards: