Meridians' Lyo-Ready mixes are glycerol-free mixes, ideal for developing ambient temperature, lyophilised, multiplex qPCR molecular diagnostic tests and are suited for high-throughput, automated platforms or microfluidic qPCR platforms requiring millisecond rehydration times.
Molecular diagnostic tests are progressively moving towards lyophilised formats. There are several advantages for this, including ambient temperature shipping and storage, extended shelf-life, reduced operating steps and potential errors and increased flexibility in sample volume. In order to be compatible with drying, however, enzyme preparations must be glycerol-free and include specialised excipients that preserve the mixture as it is exposed to various conditions including freezing, vacuum, and dehydration.
Lyo-Ready qPCR Mix 2.6x is used for the development of DNA based multiplex qPCR tests, enabling highly sensitive detection of target DNA, at a slightly higher concentration than the Lyo-Ready qPCR Mix (MDX021), the Lyo-Ready qPCR Mix 2.6x Mix is for use with microfluidic qPCR, where the initial volume needs to be smaller.
- Very fast reaction kinetics – ideal for multiplex qPCR assays and low-copy number targets
- Glycerol-free mixes are pre-formulated with a specialised blend of lyo-excipients, compatible with lyophilisation into beads or cakes
- Higher concentration makes it ideal for molecular diagnostic tests on microfluidic qPCR platforms
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