MyTaq™ One-Step RT-PCR Kit has been designed for extremely sensitive and highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube , from either total RNA or mRNA using gene-specific primers. The kit contains the latest advances in buffer chemistry, including Meridian Bioscience's ultra-pure dNTPs, together with reverse transcriptase (RT) and our new generation of very high performance, antibody-mediated hot-start DNA polymerase (MyTaq HS). This ensures that MyTaq One-Step RT-PCR Kit produces fast, highly-specific and ultrasensitive products for downstream applications. MyTaq One-Step Kit consists of reverse transcriptase, 2x MyTaq HS Mix and a potent RNase Inhibitor, RiboSafe, that are added together to create a simple to use all-in-one mix. The kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative, semi-quantitative or quantitative analysis of RNA transcription levels, and the one-step format is also perfect for the synthesis of double-stranded cDNA products for subsequent gene-expression analysis. The cDNA can be synthesised with starting amounts of RNA template from 3pg to 1µg, over a broad temperature range (up to 50°C to overcome secondary structure and GC-rich sequences), prior to heating to 95°C to inactivate reverse transcriptase and simultaneously to activate the MyTaq™ HS.
Features
- Extremely sensitive blend of RT and novel hot-start MyTaq
- Highly optimized for detection of low-copy genes
- Overcomes secondary structure in difficult and GC-rich targets
- High-quality, full-length cDNA from as little as 3pg of total RNA
- Simple to use all-in-one mix
- Applications: gene-expression analysis, transcription analysis, gene cloning, multiplex RT-PCR
- Components: MyTaq One-Step Mix (2x), Ribosafe Inhibitor (10u/µl), Reverse transcriptase, DEPC-treated Water
- Storage Conditions: The MyTaq One-Step RT-PCR Kit can be stored for 6 months at -20°C
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