Recombinant bovine/porcine FGF2-G3 154 aa protein is a thermostable engineered form of FGF-2 (bFGF). Qk081 is the 154 aa mature domain of FGF-2 (Qk056). FGF2-G3 has an increased functional half-life from 10 h (wild-type) to >7 days (FGF2-G3). Bovine FGF2-G3 is used for the development of optimized serum-free culture media for species-specific bovine (cow) cultivated meat and veterinary research applications including the expansion of bovine smooth muscles cells, bovine induced pluripotent, embryonic and mesenchymal stem cells.
Recombinant FGF2-G3 is used in B8 media (Kuo et al. 2019) and Beefy-9 media (Stout et al. 2023) for weekend free, high homogeneity induced pluripotent stem cell culture. FGF2-G3 also has applications in chemically defined stem cell and organoid culture media.
Bovine/porcine thermostable FGF2-G3 154 aa has a molecular weight of 17.2 kDa. This protein is carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency.
- High purity thermostable bovine/porcine FGF2-G3
- >98%, by SDS-PAGE quantitative densitometry
- Source: Expressed in E. coli
- 17.2 kDa (Monomer)
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Cambridge, UK
- Lyophilised from Tris/NaCl/CyS/mannitol
- Resuspend in water at >100µg/mL, prepare single-use aliquots, add carrier protein if desired, and store frozen at -20°C or -80°C