Application

B18R acts as a decoy receptor for Type I Interferons and inhibit IFN-alpha, beta, and omega and other Type I IFN receptors. For continuous expression, B18R protein is needed to supply with B18R-conditioned medium (B18R-CM) or recombinant protein. B18R RNA (human codon optimized) is required for high expression of Simplicon™ RNA at transfection and available for production of B18R-CM (conditioned medium).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Simplicon﹤TMSYMBOL>﹤/TMSY﹤WBR>MBOL> is a novel system to effect immediate high sustained protein expression of multiple genes into transfected cells without the risk of genome integration. The technology employs a single, synthetic, polycistronic, self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome1,2,3. The Simplicon﹤TMSYMBOL>﹤/TMSY﹤WBR>MBOL> RNA contains only genes encoding the VEE RNA replication machinery while the structural proteins that are required to make an infectious particle have been removed and replaced with the transgenes of interest. ﹤BR/>﹤BR/>Introduction and replication of the Simplicon﹤TMSYMBOL>﹤/TMSY﹤WBR>MBOL> RNA is expected to elicit a strong interferon response in transfected cells. To suppress the IFN responses, a Vaccinia virus protein4, B18R, is used for the original SimpliconTM technology. ﹤BR/>﹤BR/>The B18R RNA (human codon optimized) is a synthetic mRNA that is co-transfected with the Simplicon﹤TMSYMBOL>﹤/TMSY﹤WBR>MBOL> RNA to suppress the IFN responses. B18R RNA can also be used to prepare B18R conditioned medium by transfection into HFFs (Cat. No. SCC058). B18R RNA can also be used for co-transfection of any mRNAs to suppress the IFN responses and enable repeated transfection of mRNAs. Please refer to the User Guide for the Simplicon﹤TMSYMBOL>﹤/TMSY﹤WBR>MBOL> Expression System located on our website for detailed protocols.

Legal Information

SIMPLICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality

Discrete RNA band on RNA gel of appropriate size.
30-60% GFP-positive human foreskin fibroblasts (HFFs) with FACS analysis.

Storage and Stability

B18R RNA (human codon optimized): (Cat. # CS224516) One (1) vial containing 10 µL of RNA (1 µg/µL). Store at -80 °C.