General description

The Amplifluor^® UniPrimer™-fluorescein is a hairpin primer labeled with a fluorescent energy transfer pair (fluorescein and non-fluorescent quencher) and a 3′ single-stranded oligonucleotide tail and is provided at a concentration of 5.0 µM (25.0 mL).

Fluorescence (Linear:Hairpin): 39. Excitation = 495nm; Emission = 516nm.

Fluorescently labeled primers are used as probes to detect the amplified PCR product. It is used to produce the amplicon. These probes are designed such that the primer extension leads to improved fluorescence.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Amplifluor is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

5.0µM (25.0mL)