Application

Ammonium bicarbonate buffer has been used as a component of cell lysis/protein extraction buffer for proteomic sample preparation.

General description

Seppro^® Ammonium Bicarbonate Buffer is an effective buffer for protein digestion and deglycosylation reaction. It may be used to coat 2D and 3D electrophoretic gel pieces during digestion of proteins and proteomes. The ammonium bicarbonate buffer also provides moisture during enzymatic cleavage. Ammonium bicarbonate buffer regulates the microenvironmental pH of immobilized cephalosporin C acylase (CCA) for the catalysis of cephalosporin C (CPC) in a batch stirred tank reactor (STR).

Legal Information

6538 is a trademark of American Type Culture Collection

Seppro is a registered trademark of Sigma-Aldrich Co. LLC