Components

Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Control Slides, Mounting Media (Cat. No. HC08), and a user protocol.

General description

Cat. No. QIA39 is a fluorescein-conjugated version of our widely referenced TdT Colorimetric FragEL™ DNA Fragmentation Detection Kit (Cat. No. QIA33) for non-isotopic labeling. Terminal deoxynucleotidyl transferase (TdT) binds to exposed 3′-OH ends of DNA fragments generated in response to apoptotic signals and catalyzes the addition of fluorescein-labeled and unlabeled deoxynucleotides. When excited, fluorescein generates an intense signal which can be detected by fluorescence microscopy or flow cytometry. The mounting media sustains the fluorescent signal from samples labeled on slides and, therefore, aids in the morphological evaluation and characterization of normal and apoptotic cells. Non-apoptotic cells can be visualized by a DAPI filter.

Allows for detection of apoptosis at the individual cell level. Apoptosis morphology is easily detected. Detection is by flow cytometry or fluorescence microscopy. The kit mounting media permits the observance of the total cell population.

Legal Information

Parafilm is a registered trademark of Bemis Company, Inc.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Darzynkiewicz, Z., et al. 1997. Cytometry27, 1.Frey, T. 1997. Cytometry28, 253.Shapiro, H.M. 1995. Practical Flow Cytometry, Third Edition. Wiley-Liss Inc., New York, New York.Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.Gavrieli, Y., et al. 1992. J. Cell Biol.119, 493.Fawthrop, D. J., et al. 1991. Arch. Toxicol.65, 437.Martin, S.J., et al. 1990. J. Immunology145, 1859.Wyllie, A. H. 1980. Nature284, 555.Kerr, J. F. R., et al. 1972. Br. J. Cancer26, 239.

Packaging

1 ea in Fibre case

Principle

The Calbiochem™ Fluorescein-FragEL™ DNA Fragmentation Detection Kit is a non-isotopic system for the labeling of DNA breaks in apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, in cell preparations fixed on slides, or in cell suspensions. Detection and analysis of labeling is possible by flow cytometry or fluorescence microscopy.

Specifications

Assay Time: 2 h

Storage and Stability

Fluorescein-FragEL™ kit components are shipped on dry ice. Upon receipt, store kit at -20°C in a non-frost-free freezer. To avoid reagent loss in tube caps, briefly pulse spin all thawed solutions before removing caps. (see General Considerations below).

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)