Application

Polyvinylidene difluoride membranes are popular membranes for immunoblotting (Western blotting), and sequencing of proteins. The basis of protein binding to PVDF is believed to be due to hydrophobic interactions. PVDF membranes for Western blotting are available in the most commonly used 0.45 µm pore size. PVDF membranes have a greater protein binding capacity, are more durable and more solvent resistant than nitrocellulose.

Immobilon-P Polyvinylidene difluoride membrane has been used in western blotting for the transfer of proteins after sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany