Analysis Note

Positive ControlAstrocytes or cytoskeletal preparations

Application

ELISA (1:1000)Frozen Sections (1:1000, see comments)Immunoblotting (1:1000)Immunocytochemistry (1:1000, see comments)Paraffin Sections (1:1000, trypsin or heat pre-treatment required)

General description

Recognizes ~50 kDa glial fibrillary acidic protein (GFAP) in human and bovine cytoskeletal preparations.

Mouse monoclonal antibody cocktail that contains a mixture of 3 antibodies supplied as undiluted ascites. Recognizes the ~50 kDa glial fibrillary acidic protein.

This Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb is validated for use in ELISA, Frozen Sections, WB, ICC, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.

Immunogen

purified bovine GFAP protein

Bovine

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This cocktail is derived from the Bigner-Eng clones MAb1B4, MAb2E1, and MAb4A11 and provides a means for more comprehensive detection of astrocytomas than each clone alone. Each component is specific for GFAP and stains astrocytes and astrocytic processes as well as Bergman glia. Recognizes both anaplastic and reactive astrocytes by immunocytochemical staining. Does not recognize metastatic tumors and brain tumors of non-astrocytic origin, including medulloblastomas, meningiomas, choroid plexus papillomas, and schwannomas. For staining paraffin sections it is recommended that de-paraffinized sections be treated with 0.1% trypsin in 50 mM Tris-HCl, pH 7.6 for 20-30 min at 37°C or boiled in Tris-buffered saline, pH 9.0 for 15 min to expose the epitope. For immunocytochemistry or staining frozen sections, post-fixation in cold methanol or methanol/hydrogen peroxide for 10 min is required for access to the astrocytes in the sample. Antibody should be titrated for optimal results in individual systems.

Vick, W.W., et al. 1987. Acta. Cytol.31, 816.McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol.45, 692.Pegram, C.N., et al. 1985. Neurochem. Pathol.3, 119.

Packaging

100 µL in Plastic ampoule

Physical form

Undiluted ascites.

Reconstitution

Upon initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)