Lectin from Triticum vulgaris (wheat), FITC conjugate, lyophilized powder

Application

Lectin from Triticum vulgaris (wheat) has been used: to treat transfected cells for immunofluorescence to stereotaxically inject into mice for neuronal tracing in immunohistochemistry for the analysis of myocytes

For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine

Biochem/physiol Actions

Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

General description

Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.

Packaging

Package size based on protein content.

2, 5, 10 mg in glass bottle

Physical form

Contains phosphate buffer salts

Preparation Note

Conjugates are prepared from affinity purified lectin.