Application

Immobilon^®-P PVDF Membrane has been used in western blotting.

Features and Benefits

Immobilon^®-P membrane has: excellent protein retention high physical strength broad chemical compatibility suitable for a variety of staining applications and reprobing in immunodetection methods

General description

Immobilon^®-P transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 micron (µm). It is recommended for western blotting, especially for proteins greater than 20 kDa. Immobilon^®-P transfer membrane can bind a wide range of molecular weight proteins. It is used for the transfer of proteins from a variety of gel matrices. When compared to a nitrocellulose membrane, the Immobilon-P PVDF membrane has a higher signal-to-noise ratio for enhanced sensitivities, improved handling characteristics and staining capabilities and increased solvent resistance.Primary Binding Mechanisms: Electrostatic, hydrophobic;Filter Code: IPVH

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany