Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

The Biotrak^™ Cell Proliferation ELISA system, version 2 from Cytiva has been specifically designed for research purposes. Traditionally, the measurement of cell proliferation has involved the use of [3H]-thymidine to allow monitoring of DNA synthesis. The Cell Proliferation ELISA is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of 5-bromo-2′-deoxyuridine (BrdU) incorporation during DNA synthesis in proliferating cells. This technique is based on the incorporation of the pyrimidine analog BrdU instead of thymidine into the DNA of proliferating cells. The principle of the assay involves culturing cells in the presence of the respective test substances in a 96-well microplate at 37°C for 1 to 5 days.

The Biotrak^™ Cell Proliferation ELISA system, version 2 from Cytiva has been specifically designed for research purposes. Traditionally, the measurement of cell proliferation has involved the use of [3H]-thymidine to allow monitoring of DNA synthesis. The Cell Proliferation ELISA is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of 5-bromo-2’;-deoxyuridine (BrdU) incorporation during DNA synthesis in proliferating cells. This technique is based on the incorporation of the pyrimidine analog BrdU instead of thymidine into the DNA of proliferating cells. The principle of the assay involves culturing cells in the presence of the respective test substances in a 96-well microplate at 37°C for 1 to 5 days.

Features and Benefits

Nonradioactive. High level of correlation with the number of proliferating cells, with Low mean variation. As least as sensitive as [3H]-thymidine incorporation. The immunoassay can be performed in approximately 3 h. The use of a multiwell ELISA reader allows a large number ofsamples to be processed simultaneously.

General description

Designed to provide a precise, fast, and simple colorimetric assay. The technique is based upon the incorporation of the thymidine analog, 5′-bromo-2′-deoxyuridine into the DNA of proliferating cells cultured in a 96-well microplate.

Legal Information

Biotrak is a trademark of Cytiva