ECL Western Blotting Analysis System, Cytiva, RPN2108

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

ECL^™ Blotting Analysis System has been used in western blotting.

Western Blotting

Features and Benefits

Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL^™ reagents. Optimized system provides rapid, sensitive results using simple protocols. Designed for use with nitrocellulose and PVDF membranes. High sensitivity: Up to 10 times more sensitive than colorimetric methods. Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods. Easy reprobing: Save time and materials by reprobing on membranes up to 10 times. Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL, Non-radioactive-no specialized facilities needed Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide

General description

ECL^™ Western Blotting System is the world’;s most widely used and referenced chemiluminescent immunodetection system.ECL^™ Western Blotting System uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL^™ Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.Each system contains the following reagents, sufficient for detection of 1000 cm² membrane: Anti-mouse HRP conjugate, 100 µlAnti-rabbit HRP conjugate, 100 µlECL^™ Detection Reagents 1 and 2, 62.5 mL eachBlocking agent, 5 g.

Legal Information

ECL is a trademark of Cytiva