GST Bulk Kit, Cytiva, 27-4570-01, pack of 1 kit

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

GST Bulk Kit contains a 10 mL bulk pack of Glutathione Sepharose^® 4B medium, five gravity-fLow columns, IPTG, and GST Buffer Kit. GST-tagged Proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of Glutathione Sepharose^® 4B, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used.

Features and Benefits

For rapid purification of GST-tagged Proteins using gravity flow columns or using batch purification with high specificity. Preserves Protein antigenicity and function. Yields up to 50 mg of pure Protein.

General description

GST bulk kit is designed for small-scale manual purification of GST-tagged proteins using either column chromatography or batch method. Facilitates optimization studies of expression, solubility conditions and purification parameters of GST-tagged proteins.

Legal Information

Sepharose is a registered trademark of Cytiva