Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Application
The multimodal functionality gives a different selectivity compared with traditional ion exchangers including binding of Proteins regardless of ionic strength of the loading material . This means that the medium may be used for direct load of clarified feedstocks, without prior dilution to reduce the conductivity of the starting material. To reduce tedious sample preparation, the top filter in HiTrap® Capto™ MMC has been optimized for loading sonicated unclarified cell lysate directly on the column.The Capto™ MMC ligand may interact with Proteins in many different ways. It contains a carboxyl group, and thus it is a weak cation exchanger. However, in addition to the ionic interactions several other types of interactions are involved, including hydrogen bonding and hydrophobic interaction.
Features and Benefits
High dynamic binding capacity at high conductivity. Selectivity allows binding in the presence of salt. High volume throughput. HiTrap® Capto™ MMC can be used to bind Proteins at the conductivity of the feed material and to solve specific purification challenges.
General description
HiTrap® Capto™ MMC is prepacked with Capto™ MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.
Legal Information
Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Storage and Stability
Store at 4 to 30 °C (20% Ethanol)
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