G5166-100UN Display Image

PNGase F from Elizabethkingia miricola, buffered aqueous solution

Code: G5166-100UN D2-231

Application

Used to deglycosylate protein.

PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.

Biochem/...


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Your Price
€614.91 EACH

Application

Used to deglycosylate protein.

PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

General description

PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.

Physical form

Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA

Unit Definition

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

biological sourcebacterial (Elizabethkingia miricola)
conjugate(N-linked)
formbuffered aqueous solution
mol wt36 kDa
Quality Level200
shipped inwet ice
specific activity≥20000 units/mg protein
storage temp.2-8°C
Code
Description
Unit Size
List Price
Qty
G5166-50UN
Unit:EACH
List Price: €360.25
Source:List Price
ADD
Cas Number83534-39-8
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