Application

The CHEMICON^® Alpha/Beta Integrin-Mediated Cell Adhesion Array Kit can be used for assessing the presence or absence of specific integrins on the cell surface. This assay can substitute for FACS analysis (6) and is useful for screening human adherent cells for cell surface integrins or for monitoring in vitro cell differentiation or genetic modification of cells.

For Research Use Only. Not for use in diagnostic procedures.

Research CategoryCell Structure

The Alpha/Beta Integrin-Mediated Cell Adhesion Array Kit can be used for assessing the presence or absence of specific integrins on the cell surface.

Components

Alpha Integrin Array Plate: (PN: 90599) One 96-well plate with 12 strips. Each strip contains one well each of the mouse anti-alpha integrin monoclonal antibodies: (alpha1, alpha2, alpha3, alpha4, alpha5, alphaV, alphavbeta3), and one goat anti-mouse negative well. See the Alpha Integrin plate layout on the insert data sheet.Beta Integrin Array Plate: (PN: 90600) One 96-well plate with 12 strips. Each strip contains one well each of the mouse anti-beta integrin monoclonal antibodies: (beta1, beta2, beta3, beta4, beta6, alphaVbeta5, and alpha5beta1), and one goat anti-mouse negative well. See the Beta Integrin plate layout on the insert data sheet.4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mLCyQuant GR Dye™: (Part No. 90132) Two vials - 75 µL eachAssay Buffer: (Part No. 90601) Two bottles - 100 mL each

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

GR is a trademark of Sigma-Aldrich Co. LLC

Packaging

96 wells each

2 plates in 96 wells each

Storage and Stability

The Alpha and Beta Integrin Array Plates can be stored at 2° to 8°C in the foil pouch up to their expiration date. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed. Keep the remaining kit components at 2° to 8°C.

Precautions

No data is available on the biological toxicity of CyQuant GR^™ dye. This reagent binds to nucleic acids, and as such, should be treated as a potential mutagen which may cause cancer and heritable genetic damage. Handle with caution. The DMSO stock solution should be handled with special caution as DMSO can facilitate the entry of organic molecules into tissues.