Application

The FLAG Purification Kit utilizes CelLytic^™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG^® M2 affinity gel for affinity purification of active FLAG fusion proteins.Browse additional application references in our FLAG^® Literature portal.

Features and Benefits

Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins. ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations. Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.

Packaging

Sufficient for 3-5 uses of 1-ml affinity purification column.