Application

CelLytic^™ Y Cell Lysis Reagent has been used in lysis of yeast cells expressing DNA repair, Rad protein, heat shock protein (ssa1) and human interleukin 4 (hIL4).

Features and Benefits

Fast: Cell lysis in just 20 minutes Efficient: Three times more efficient than glass beads Flexible: Lysis is enhanced by the addition of reducing reagent (DTT) Gentle: Non-denaturing - preserves biological activity

General description

CelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC