Application

Biuret reagent has been used for protein quantification of high-pressure thermal hydrolysis lysate(HPTH), oyster hemolymph and Clostridium cellulovorans(CBDclos) aggregates.

General description

Biuret reagent is used for measuring total protein concentration. It comprises of copper II (cupric ion) alkaline solution, which interacts with the peptide bond to form a blue adduct which is measured at 540nm. Biuret method is less sensitive when compared to other protein estimation methods like Lowry and Folin-Ciocalteu techniques.