Application

For purification of N-terminal FLAG fusion proteins. Since binding is Ca²⁺-dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3. ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins.immunoprecipitation (IP): suitableElution - FLAG peptide, Glycine, pH 3.5 EDTABrowse additional application references in our FLAG^® Literature portal.

Features and Benefits

Typically purify fusion proteins from crude lysates to single band purity in just one chromatography step. Fusion protein may be eluted from affinity resin by mild elution with EDTA. A solution of FLAG peptide can be used for gentle, non-denaturing elution of FLAG fusion proteins.

General description

ANTI-FLAG^® M1 Agarose Affinity Gel is a purified mouse IgG2B monoclonal antibody covalently attached to agarose.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

ANTI-FLAG^® M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.

Physical form

Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide

Specificity

Binding specificity: Free N-Terminus of FLAG sequenceN-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C