General description

A modified biotin conjugate in which biotin is linked to a bis-aryl hydrazone chromophore and aromatic succinimidyl ester via a long-chain PEG4 linker. The bis-aryl hydrazone chromophore allows for direct spectrophotometric quantitation of total incorporated biotin. The long-chain PEG4 linker preserves biotin/avidin affinity as well as increases solubility. The aromatic succinimidyl ester allows for higher efficiency modification of amines in aqueous buffers.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Steinberg-Tatman, G., et al. 2006. Bioconjug. Chem.17, 841.Kline, M.C., et al. 2005. J. Forensic Sci.50, 1.Kozlov, I.A., et al. 2004. Biopolymers73, 621.Chen, Y., et al. 2004. Chem. Commun.24, 2804.Kozlov, I.A., et al. 2004. Biopolymers73, 621.Zhong, X.B., et al. 2003. Proc. Natl. Acad. Sci. USA100, 11559.Hartmann, D.M., et al. 2002. Journal of Materials Research17, 473.

Packaging

10 mg in Plastic ampoule

Reconstitution

Following reconstitution, purge with inert gas, aliquot, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)