Application

CHAPS 100 mM solution has been used as a component of lysis buffer for the extraction of proteins from goat endometrial epithelial cells (EECs) and liver tissue.

Biochem/physiol Actions

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes).CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.

General description

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.