Analysis Note

ControlControls
Includes normal rabbit IgG and primers specific for a human prostate-specific antigen enhancer region.

Application

Research Sub CategoryTranscription Factors

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from hormone starved (72H), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (~1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No.CS207349) and the Magna ChIP™ HiSens (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340) as a positive locus, and ChIP Primers, β-globin (Part No. CS200591) as a negative locus.
Please refer to the Magna ChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens(Cat. # 17-10461) protocol for experimental details.

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from hormone starved (72H), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (1e5 and 1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No. CS207349) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340).
Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.

Western Blotting Analysis:
0.04 µg/mL of this antibody detected MED1 on 5, 15 and 50 µg of HeLa whole cell lysate.

Research CategoryEpigenetics & Nuclear Function

Use ChIPAb+ MED1 ChIP Validated rabbit polyclonal antibody for the detection of MED also known as Mediator of RNA polymerase II transcription subunit 1 & CRSP1/TRAP220.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ MED1 (CRSP1/TRAP220) set includes a MED1 antibody, a Normal Rabbit IgG, and positive control primers which amplify 85 bp of a human prostate-specific antigen (PSA) enhancer region. The MED1 antibody and negative control IgG are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of MED1 associated chromatin.

Immunogen

Recombinant protein corresponding to human MED1 (a.a. 1525-1581).

Epitope: Human MED1 (a.a. 1525-1581).

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Packaging

25 assays per set. Recommended use: 2.0 µg of antibody per chromatin immunoprecipitation (dependent upon biological context).

Physical form

Anti-MED1 (Rabbit Polyclonal), Part No. CS207349. One vial containing 50 µg affinity purified rabbit polyclonal in 72 uL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7 mg/mL final). Store at -20°C.

Normal Rabbit IgG, Part No. PP64B. One vial containing 125 µg of purified Rabbit IgG in 125 µL storage buffer containing 0.05% sodium azide. Store at -20°C.

ChIP Primers, PSA. Part No. CS207340. One vial containing 75 µL of 5 µM of each primer specific for human prostate-specific antigen enhancer region (chr19:51354179-51354263, hg19 build). Store at -20°C.
FOR: GCC TGG ATC TGA GAG AGA TAT CAT C
REV: ACA CCT TTT TTT TTC TGG ATT GTT G

Quality

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from hormone starved (72h), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (~1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No.CS207349) and the Magna ChIP^® HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340).
Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.

Specificity

Wide species reactivity expected based upon conservation of immunogen sequence.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~168 kDa based on sequence. Higher mw up to 220 kDa observed on western blots.