Application

The Magna ChIRP RNA Interactome Kit allows analysis, and mapping of regions of chromatin interacting with chromatin-associated RNAs. Based on the ChIRP method (chromatin isolation by RNA purification), this probe-based capture assay enables discovery of RNA-associated DNA sequences and proteins.

Components

10X Glycine ;Lysis Buffer;Hybridization Buffer (without 15% formamide) ;Wash Buffer;Proteinase K Buffer (for DNA);Proteinase K Buffer (for RNA);DNA Elution Buffer;0.5M EDTA;Streptavidin Magnetic Beads;RNase Inhibitor;Protease Inhibitor Cocktail III;Proteinase K Solution;DNase I RNase free;DNase I Reaction Buffer;RNase A (20 mg/mL);RNase H (10U/ µL);Magna ChIRP Negative Control Probe Set (LacZ)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Magna ChIRP RNA Interactome Kit uses the ChIRP method (Chromatin Isolation by RNA Purification) to enable identification and analysis of regions of chromatin that interact with a particular chromatin-associated RNA. This novel anti-sense probe-based capture strategy uses cross-linked chromatin to allow unbiased discovery of RNA-associated DNA sequences and proteins. To perform ChIRP two pools consisting of mulitple biotinylated oligonucleotide probes complementary to the RNA of interest are used (see figure 1). These probe sets are combined with chromatin and hybridized to the chromatin-associated RNA. Complexes containing biotinylated-probes bound to the chromatin-associated RNA are then isolated using streptavidin magnetic beads. DNA or RNA can then be recovered and analyzed by quantitative PCR or next generation sequencing (ChIRP-seq). Alternatively, proteomic analysis of the recovered chromatin can be performed.

Legal Information

MAGNA CHIRP is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

Kit capacity: 12 Chromatin Isolation by RNA Purification Assays (includes negative control probes)