Application

Lot # R0803D0002: 12.5 µg NAP1 has been successfully used for 1 chromatin assembly reaction with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410).

General description

Nucleosome assembly proteins (NAP) are involved in moving histones into the nucleus, nucleosome assembly and chromatin fluidity. They affect the transcription of many genes.

N-terminal 6×His-tagged Drosophila NAP1 expressed in E. coli.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Specific Activity: Varies with lot

Varies with individual lot. Lot R0803D0002 has purity 90% by SDS-PAGE and Coomassie blue staining

Physical form

10 mM K-HEPES pH 7.6, 10 mM KCl, 50 mM NaCl, 0.1 mM EDTA, 10%(v/v) glycerol, 0.01% NP-40, 0.2 mM PMSF, 1mM DTT. Frozen solution.

Format: Purified

Quality

Activity is routinely tested with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410).

Storage and Stability

Stable for 6 months at -80°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.