Application

Suitable for in vitro chromatin assembly and enzyme activity assays

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Human native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease, and purified by glycerol gradient and ion exchange chromatography.

Nucleosomes are the basic units of chromatin, and are comprised of approximately 147 bp of DNA spooled around a central octamer of core histones, H2A, H2B, H3, and H4. The arrangement of nucleosomes in chromatin can be altered by post-translational modifications (PTM) occurring on the C- and N-terminal tails of the core histones. Histone modifications can either provide or hinder access of key transcriptional complexes to DNA sequences and can regulate the expression of genes. These effects can be deciphered by applications such as chromatin immunoprecipiation (ChIP), which requires high quality chromatin, and essential tools such as histone proteins and appropriate enzymes, to assemble chromatin in vitro.

Product Source: Human

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

20 µg of mononucleosomes in buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 0.5 mM PMSF,1 mM DTT, and 50% glycerol.

Storage and Stability

Stable at -80°C for 1 year from date of receipt.