Application

Research CategoryEpigenetics & Nuclear Function

HDAC deacetylase assays

Human recombinant SIRT5 (His-tag) expressed in E. Coli.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: Recombinant residues 37-310 of human SIRT5 isoform 1, with an N-terminal His-tag

Human recombinant SIRT5 (His-tag) expressed in E. Coli

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Format: Purified

In liquid containing 25 mM Tris, pH 7.5, 100 mM NaCl, 5 mM DTT, containing 10% glycerol. Frozen solution.

Storage and Stability

Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the SIRT5 into separate tubes before storing at -80°C.