Analysis Note

Low-end CV% Range: 2 - 6%|Low-end CV% Average: 4%

Application

Research CategoryMetabolism

The sensitivity of Single Molecule Counting (SMC^®) technology gives the SMC^® Active GLP-1 Immunoassay Kit unparalleled performance in the quantification of active GLP-1 in human EDTA plasma.

Research Sub CategoryMetabolic Disorders

Used to detect/quantify:GLP-1 (Active)

Components

Active GLP-1 Coated Beads Vial 1Active GLP-1 Coated Beads Vial 2Active GLP-1 Standard DiluentGLP-1 Assay BufferGLP-1 StandardActive GLP-1 Detection Antibody10X Wash BufferElution Buffer BBuffer DSMC^® Active GLP-1 Immunoassay Kit Instructions

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

General description

Glucagon-Like Peptide-1 (GLP-1) is a member of the incretin family of hormones, which are released by the gut into the bloodstream in response to food. Post-translational cleavage of the peptide produces active GLP-1, which is then rapidly degraded by protease dipeptidyl peptidase-4 (DPP-4). When blood glucose level is high, active GLP-1 enhances insulin secretion and also suppresses the release of glucagon. In addition, GLP-1 stimulates the brain to trigger feelings of satiety and regulates the rate of gastric emptying.

The SMC^® Active GLP-1 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Legal Information

SMC is a registered trademark of Singulex