Analysis Note

Color (visual): white to yellow to beigeAppearance of substance (visual): beadsLoading (determined by elemental analysis for sulfur after derivatization with KSAc): 0.40 - 1.00 mmol/gSwelling Volume (in CH₂Cl₂): lot specific resultThe polymer matrix is copoly (styrene-1 % DVB), 100 -200 mesh (75 - 150 µm)

General description

Merrifield resin was the standard support for the synthesis of peptide acids by Boc SPPS [1, 2]. In peptide chemistry, it is now really only used in the synthesis of small to medium sized peptides, as the benzylic ester resin linkage is not completely stable towards repetitive treatment with TFA.

Attachment of the C-terminal residue is generally achieved by heating the resin in DMF with the appropriate amino acid cesium salt in the presence of KI [3]; although, Me₄N salts [4], sodium salts [5] in THF with Bu₄NF catalysis [6] and zinc salts in EtOH have also been used [7].

Cleavage is normally effected by treatment of resin with HF or TFMSA, or by hydrogenolysis [8]. Alcohols can be released using reducing agents such as DIBAL [6] or LiBH₄ [9]. Methyl esters can be produced by transesterification with methoxide [10].

The product number for this product was previously 01-64-0008.

To obtain a certificate of analysis (CoA) of a lot that begins with the letter “A”, please select the option in the right hand menu “Request a COA for Lot#s starting with A”.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 01-64-0008