Analysis Note

Color (visual): white to slight yellow to light redAppearance of substance (visual): powderIdentity (IR): passes testPurity (TLC(CMA1)): ≥ 98 %Assay (HPLC, area%): ≥ 95.0 % (a/a)Solubility (1 mmole in 2 ml DMF): clearly solubleFor TLC-Systems see our General Catalog

General description

Fmoc-ACA-OH is a useful tool for the SPPS of AMC fluorogenic protease substrates. Fmoc-ACA-OH [1] must be first loaded onto a Wang-type resin. Any unreacted linker hydroxyls must be capped using acetic anhydride. Following Fmoc removal, the first amino acid should be coupled withy HATU/collidine [2]. Insertion of ACA into the middle of a peptide provides fluorogenic substrates for endopeptidases [3]. Treatment of the ACC peptide obtained after TFA cleavage with aqueous base causes facile decarboxylation and formation of the AMC peptide.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

250 mg in Plastic bottle

1 g in Glass bottle