Analysis Note

Colour (visual): white to off whiteAppearance of substance (visual): powderColour index (0,5 M in DMF): ≤ 150 HazenIdentity (IR): passes testEnantiomeric purity: ≥ 99.7 % (a/a)Purity (HPLC): ≥ 99.0 % (a/a)Fmoc--Ala-OH (HPLC): ≤ 0.1 % (a/a)Fmoc--Ala-Asn (Trt)-OH (HPLC): ≤ 0.1 % (a/a)Fmoc-Asn(Trt)-Asn(Trt)-OH (HPLC): ≤ 0.1 % (a/a)Fmoc-Asn-OH (HPLC): ≤ 0.1 % (a/a)Assay free amino acid (GC): ≤ 0.2 %Purity (TLC(011A)): ≥ 98 %Purity (TLC(0811)): ≥ 98 %Solubility (25 mmole in 50 ml DMF): clearly solubleAssay (acidimetric): ≥ 98.0 %Water (K. F.): ≤ 2.0 %Ethyl acetate (HS-GC): ≤ 0.5 ¬etate (IC): ≤ 0.05 %For TLC-Systems see our General Catalog

General description

Fmoc-Asn(Trt)-OH has good solubility properties in most organic solvents, and its use has been shown to result in significantly purer peptides than other derivatives used for the introduction of Asn [1, 2]. Coupling can be performed by standard procedures. The trityl group is normally removed by 95% TFA in 1-3 hours, with no alkylation of Trp residues. When Asn(Trt) is the N-terminal residue, the reaction time may need to be extended to ensure complete deprotection [3].

The product number for this product was previously 04-12-1089.

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Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-12-1089

Packaging

1 kg in Plastic bottle