Analysis Note

Colour (visual): white to off whiteAppearance of substance (visual): powderColour index (0,5 M in DMF): ≤ 150 HazenIdentity (IR): passes testEnantiomeric purity: ≥ 99.5 % (a/a)Purity (HPLC): ≥ 99.0 % (a/a)Fmoc--Ala-OH (HPLC): ≤ 0.1 % (a/a)Fmoc--Ala-Cys (Trt) -OH (HPLC): ≤ 0.1 % (a/a)Fmoc-Cys(Trt)-Cys(Trt)-OH (HPLC): ≤ 0.1 % (a/a)Fmoc-Cys-OH (HPLC): ≤ 0.1 % (a/a)Assay free amino acid (HPLC): ≤ 0.2 %Purity (TLC(011A)): ≥ 98 %Purity (TLC(0811)): ≥ 98 %Solubility (1 mmole in 2 ml DMF): clearly solubleAssay (acidimetric): ≥ 98.0 %Water (K. F.): ≤ 2.0 %Ethyl acetate (HS-GC): ≤ 0.5 ¬etate (IC): ≤ 0.02 %For TLC-Systems see our General Catalog

General description

The standard derivative for Fmoc SPPS of peptides containing Cys [1]. The Trt group is removed with 95% TFA containing 1-5% TIS. Ideally, this derivative should be introduced using the symmetrical anhydride or DIPCDI/HOBt activation [2,3] to minimize enantiomerization. If activation with uronium or phosphonium reagents, such as HBTU or PyBOP^®, is to be employed, it is strongly recommended that collidine is used as the base [4], as this has been shown to significantly reduce loss of optical integrity during coupling.

The product number for this product was previously 04-12-1018.

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Legal Information

PyBOP is a registered trademark of Merck KGaA, Darmstadt, Germany

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-12-1018

Packaging

1 kg in Plastic bottle