Nippon Genetics FastGene Scriptase III cDNA Kit - 100 Reactions

The Scriptase III is an enhanced variant of the Murine Leukemia Virus (MuLV) reverse transcriptase. Due to specific mutations in the RNase H domain of the reverse transcriptase, cDNA can be synthesised even from very small amounts of RNA. Scriptase III has additional mutations that make it even more robust and thermostable. It can therefore operate with maximum activity up to a temperature of 55°C. At these elevated temperatures, highly structured RNAs can easily unfold, allowing them to serve as templates for reverse transcription. It is the superior choice for complex tasks, such as Real-Time PCR and NGS.

• Contains all the components for efficient and reliable reverse transcription
• Designed to simplify your workflow, the kit includes all the essential components for a robust cDNA synthesis reaction
• The Scriptase III enzyme, together with optimised buffers, dNTPs, inhibitors and primers ensures high yields, exceptional sensitivity, and reproducibility
• FastGene® Scriptase III cDNA Kit components:
• Scriptase III (200 units/µL)
• 10X RT buffer
• 25mM MgCl₂
• 0.1M DTT
• dNTP mixture (10mM each)
• RNase inhibitor
• Sterile water (RNase free)
• Oligo dT (80µM)
• Random hexamer (50ng/µL)