Nippon Genetics FastGene Scriptase Basic cDNA Kit - 100 Reactions

Code: PCR2029 D2-0

The FastGene® Scriptase Basic is an enhanced version of the Murine Leukemia Virus (MuLV) reverse transcriptase. Hence, like the wild type, it has the ability to synthesise a cDNA strand, a red...


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€269.10 EACH
€330.99 inc. VAT

The FastGene® Scriptase Basic is an enhanced version of the Murine Leukemia Virus (MuLV) reverse transcriptase. Hence, like the wild type, it has the ability to synthesise a cDNA strand, a reduced RNase H activity and processitivity. The robustness however was greatly increased. It is the perfect enzyme for large RNA quantities and easy templates.

  • No inhibition – even at large concentration: The special buffer formulation permits a high RNA concentration. Other reverse transcriptases are not able to process such large quantities.
  • Designed for Endpoint RT-PCR: The FastGene® Scriptase Basic was designed for large RNA quantities, typically used in an endpoint RT-PCR. Nonetheless, it is also able to process lower RNA concentrations.
  • Enzyme only or cDNA synthesis kit: The FastGene® Scriptase Basic is available as enzyme only (PCR2026), containing the enzyme, buffer and dNTPs as well as a cDNA synthesis kit (PCR2029) which contains the components of the enzyme plus oligo dTs, random hexamers, and RNase inhibitor
  • 20000 units at 200 units/µL (for 100 reactions)
  • Buffer (10x FastGene® Scriptase Basic Buffer), dNTP mixture (2mM each) and sterile water (RNase-free) included
  • Oligo dTs, random hexamers and RNases inhibitor included (only for PCR2029)
  • Endonuclease-free, exonuclease-free and RNase-free
  • Reverse transcriptase for the quantification of gene expression
  • For high DNA concentrations

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