The FastGene® Scriptase Basic is an enhanced version of the Murine Leukemia Virus (MuLV) reverse transcriptase. Hence, like the wild type, it has the ability to synthesise a cDNA strand, a reduced RNase H activity and processitivity. The robustness however was greatly increased. It is the perfect enzyme for large RNA quantities and easy templates.
- No inhibition – even at large concentration: The special buffer formulation permits a high RNA concentration. Other reverse transcriptases are not able to process such large quantities.
- Designed for Endpoint RT-PCR: The FastGene® Scriptase Basic was designed for large RNA quantities, typically used in an endpoint RT-PCR. Nonetheless, it is also able to process lower RNA concentrations.
- Enzyme only or cDNA synthesis kit: The FastGene® Scriptase Basic is available as enzyme only (PCR2026), containing the enzyme, buffer and dNTPs as well as a cDNA synthesis kit (PCR2029) which contains the components of the enzyme plus oligo dTs, random hexamers, and RNase inhibitor
- 20000 units at 200 units/µL (for 100 reactions)
- Buffer (10x FastGene® Scriptase Basic Buffer), dNTP mixture (2mM each) and sterile water (RNase-free) included
- Oligo dTs, random hexamers and RNases inhibitor included (only for PCR2029)
- Endonuclease-free, exonuclease-free and RNase-free
- Reverse transcriptase for the quantification of gene expression
- For high DNA concentrations
This product has met the following criteria to qualify for the following awards: