Meridian’s Glycerol-Free Phi29 DNA Polymerase (HC), (MDX240) is a highly processive enzyme with strong strand displacement activity, enabling efficient isothermal DNA amplification from low-copy samples. Its 3′→5′ exonuclease (proofreading) activity ensures high-fidelity amplification, while its exceptional processivity allows for the synthesis of ultra-long, high molecular weight DNA products.

This high-concentration, glycerol-free formulation is optimized for multiple displacement amplification (MDA) and rolling circle amplification (RCA), facilitating whole genome amplification, single-cell genomics, and molecular diagnostics. The enzyme’s strong strand displacement activity ensures robust performance, even from low-copy templates.

Glycerol-Free Phi29 DNA Polymerase (HC) does not contain glycerol and has been optimized to deliver robust performance, making it ideal for the development of diagnostic assays in both liquid and lyophilised formats.

• Strong strand displacement activity enables efficient isothermal amplification at 30°C
• High processivity, synthesising products up to 70 kb in length
• Exonuclease Activity (3' → 5') providing proofreading capability for high-fidelity replication
• High yields, ideal for amplification from small amounts of template