Glycerol-Free DNA Pol I Klenow Fragment exo- (HC) is a high concentration N-terminal truncation of E.coli DNA Polymerase I which retains polymerase activity, but has lost 5’-3’ exonuclease activity and has mutations (D355A, E357A) which eliminates the 3’-5’ exonuclease activity. It is designed for dA and dT tailing for adapter ligation in next generation sequencing library preparation.

Glycerol-Free DNA Pol I Klenow Fragment exo- (HC) is as a glycerol-free, high concentration enzyme (50 U/L) and is mainly used in next-generation sequencing (NGS) library preparation, where it can be used separately or as part of an End-Repair Mix, for double stranded DNA blunting by 3’ overhang removal and fill-in of 5’ overhangs for subsequent ligation of adaptors by T4 DNA Ligase. The enzyme displays moderate strand displacement activity and can also be used to generate probes using random primers, dideoxy sequencing and random priming labelling.

Glycerol-Free DNA Pol I Klenow Fragment exo- (HC) is in a glycerol-free storage buffer and is supplied with a Lyo-Ready Klenow Reaction Buffer (5x) and has been optimised to deliver excellent, stable performance without glycerol, allowing you to deliver reliable results with the added capability for lyophilisation. This gives you the flexibility to confidently store mixtures at ambient temperature or produce diagnostic assays that rely on miniaturised reaction components, such as point-of-care NGS.

• dA- and dT-tailing for next-generation sequencing (NGS) adapter ligation
• Labelling by fill-in 5'-overhangs of dsDNA
• DNA sequencing by the Sanger method
• Isothermal DNA amplification SDA (Strand Displacement Amplification)