T4 Polynucleotide Kinase is widely used to phosphorylate the 5’ end of both DNA and RNA during labelling and ligation by catalysing the transfer of the γ-phosphate from ATP to the 5´ hydroxyl of single stranded and double stranded DNA, RNA, and nucleoside 3´ monophosphates. T4 Polynucleotide Kinase also catalyses the hydrolytic removal of the 3´ phosphate from 3´-phosphoryl polynucleotides, deoxyribonucleoside 3´ monophosphates, and deoxyribonucleoside-3´,5´-diphosphates to form a 3´-hydroxyl group.
Glycerol-Free T4 Polynucleotide Kinase (HC) is supplied at 20 U/µL and is mainly used in next-generation sequencing (NGS) library preparation, where it can be used separately or as part of an End-Repair Mix, where phosphates are added to the 5′-hydroxyl terminus of double stranded DNA for subsequent ligation of adaptors by T4 DNA Ligase. Glycerol-Free T4 Polynucleotide Kinase (HC) is in a glycerol-free storage buffer and is supplied with a Lyo-Ready reaction buffer and has been optimised to deliver excellent, stable performance without glycerol, allowing you to deliver reliable results with the added capability for lyophilisation. This gives you the flexibility to confidently store mixtures at ambient temperature or produce diagnostic assays that rely on miniaturised reaction components, such as point-of-care NGS.
- 5' phosphorylation for subsequent ligation for NGS
- End-labelling for probes and oligodeoxynucleotide primers
- Removes 3′ phosphoryl groups from DNA and RNA
- Glycerol-free, for lyophilisation and ambient temperature storage
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