Meridian Bioscience Glycerol-Free HS Tth DNA Polymerase (HC) - 100000 Units

Thermus thermophilus (Tth) DNA Polymerase is a thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity. The enzyme is a highly processive 5′-3′ DNA polymerase and lacks 3′-5′ exonuclease activity (proof reading). It possesses a very efficient intrinsic reverse transcriptase (RT) activity in the presence of manganese ions and unlike other RT enzymes, it is not associated with RNase H activity.

Glycerol-Free Tth DNA Polymerase (HC) is a high concentration (200 U/µL) thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity. The elevated temperatures of Tth DNA Polymerase activity overcomes the problems posed by RNA secondary structure. Resulting cDNA can be amplified by PCR using the same enzyme in the presence of Mg²⁺ ions. The ability of Tth DNA Polymerase to perform both reverse transcription and DNA amplification at elevated temperatures allows this enzyme to be used for quantitative RT-PCR, cloning, and gene expression analysis of cellular and viral RNA.

Glycerol-Free HS Tth DNA Polymerase uses antibody-mediated hot start technology that enhances the specificity and sensitivity of PCR. It is supplied with a 5x Reaction Buffer that contains dNTPs and excipients required for lyophilisation. Glycerol-Free HS Tth DNA Polymerase enables flexible and scalable reaction volumes and is suitable for applications such as fast RT-qPCR amplification of difficult templates from crude samples.

• PCR amplification of DNA and one-step RT-PCR of RNA
• PCR amplification of RNA and DNA templates having a high degree of secondary structure present in RNA
• No association with RNase H activity
• Higher tolerance to PCR inhibitors